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Related Experiment Video

Updated: May 25, 2025

Microfluidics in Assessing Platelet Function
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Cold vs. Room Temperature: A Comparative Analysis of Platelet Functionality in Cold Storage.

Panagiotis V Drossos1, Sotirios P Fortis1, Alkmini T Anastasiadi2

  • 1Laboratory of Reliability and Quality Control in Laboratory Hematology (HemQcR), Department of Biomedical Sciences, School of Health & Caring Sciences, University of West Attica (UniWA), 12243 Egaleo, Greece.

Biomedicines
|February 26, 2025
PubMed
Summary

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Cold storage preserves platelet function better than room temperature storage, enhancing clot formation and stability. This suggests cold storage may improve transfusion therapy and extend platelet shelf-life.

Area of Science:

  • Transfusion Medicine
  • Hematology
  • Biotechnology

Background:

  • Platelet functionality during cold storage is debated.
  • Investigating temperature's impact on stored platelet hemostatic properties is crucial.

Purpose of the Study:

  • To evaluate the effect of cold versus room temperature storage on platelet hemostatic function.
  • To compare metabolic, physiological, and vesiculation parameters of platelets under different storage conditions.

Main Methods:

  • Ten pooled platelet units were stored at 4°C and 22°C.
  • In vitro analysis included metabolic, physiological, vesiculation, and hemostatic parameters (ROTEM®) on storage days 1, 5, 10, and 15.

Main Results:

  • Cold-stored platelets showed better integrity (lower LDH).
Keywords:
ROTEM® analysiscold-stored plateletsplatelet functionalitystorage conditions

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  • Cold storage maintained superior clot initiation, elasticity, strength, and firmness, especially at extended storage.
  • Cold-stored platelets exhibited enhanced aggregation and procoagulant activity of extracellular vesicles.

    • Conclusions:

    • Cold storage significantly outperforms room temperature storage for preserving platelet hemostatic function.
    • Cold storage may enhance platelet transfusion therapy and extend shelf-life.
    • Further clinical studies are needed to confirm the clinical relevance.