Related Experiment Video
Updated: May 25, 2025
09:58
RhoC GTPase Activation Assay
Published on: August 22, 2010
12.4K
A Protein Kinase Cε/Protein Kinase D3 Signalling Axis Modulates RhoA Activity During Cytokinesis
Ursula Braun1, Michael Leitges1
1Division of BioMedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, 300 Prince Philip Drive, St. John's, NL A1B 3V6, Canada.
Biomedicines
|February 26, 2025
Summary
Protein kinase D3 (PKD3) regulates cell division by controlling RhoA activity. Its depletion causes failed cell abscission and multinucleated cells, revealing a new PKCε/PKD3 pathway in cytokinesis.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Protein kinase D3 (PKD3) is part of the PKD family involved in intracellular signaling.
- Specific functions and isoform roles of PKD3 in vivo are not well-defined.
Purpose of the Study:
- To investigate the role of PKD3 in cytokinesis.
- To elucidate the signaling pathway involving PKD3 in cell division.
Main Methods:
- Utilized PKD3-depleted mouse embryonic fibroblast cells.
- Performed cell culture-based assays and fluorescence microscopy.
Main Results:
- PKD3 modulates RhoA activity via a PKCε/PKD3 signaling axis during cytokinesis.
- PKD3 depletion results in sustained RhoA activity post-immortalization.
Conclusions:
- PKD3 depletion disrupts cytokinesis, leading to failed abscission and multinucleation.
- Identified a novel PKCε/PKD3 pathway regulating cytokinesis.
Related Concept Videos
01:24Small GTPases - Ras and Rho
3.9K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
3.9K
01:21Cell Polarization by Rho Proteins
2.6K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
2.6K
01:22PI3K/mTOR/AKT Signaling Pathway
3.4K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.4K
01:20The JAK-STAT Signaling Pathway
8.6K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
8.6K
01:07MAPK Signaling Cascades
5.1K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.1K
01:22Amplifying Signals via Enzymatic Cascade
8.2K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
8.2K