Bacterial Communities from the Copper Mine of Wettelrode (Germany)

Bacterial communities from three different sampling sites of a copper mine tunnel were characterized by 16S rRNA sequencing (NGS). A high presence of halophilic bacteria was confirmed by comparison with literature data and with reference samples from other highly salt-exposed soils. Among others, high read numbers of Gracilimonas, Kangiella, Limibacillus, Marinobacter, Woseia, and uncultivated strains of Actinomarinales, Gammaproteobacterium AT-s16, Actinobacteria 0319-7L14, and Thiotrichaceae were found. The community in a sample from the surface of the copper seam was significantly different from the community composition of a sample from the mine tunnel floor. The specificity in the appearance and in the abundance of special bacterial types (for example, Thiogranum, Thiohalophilus, Sulfuriflexus, Sedimenticolaceae, Desulfomonile, Desulfosporosinus, and Cand. Thiobios) can be partially explained by the different local conditions for sulfur-related metabolisms at the sampling sites.