Anas barbariae 200K Modulates Cell Stiffness and Oxidative Stress in Microglial Cells In Vitro

Anas barbariae 200K, a homeopathic medicine, is traditionally used for influenza-like illnesses. We investigated the effects of Anas barbariae 200K on microglial cells, a subpopulation of macrophages specific to the central nervous system often used to study the inflammatory processes and oxidative stress generated during influenza-like episodes. The study demonstrates the effect of Anas barbariae 200K on cell stiffness and the reactive oxygen species production using atomic force microscopy and fluorescence microscopy techniques, respectively. Our results showed that Anas barbariae 200K rapidly increased cell stiffness in resting cells by 41% compared with the vehicle. In inflamed cells, cell stiffness was decreased by 21% when treated with Anas barbariae 200K compared with the vehicle. Finally, Anas barbariae 200K caused a reorganisation of filamentous actin, with marked relocation of actin at the cell extremities. Moreover, Anas barbariae 200K significantly decreased the reactive oxygen species (ROS) production in inflamed microglial cells by 40% (total intracellular ROS) and by 67% (mitochondrial ROS) compared with the vehicle. These results strongly suggest an effect of Anas barbariae 200K at a cellular level on cell stiffness and actin cytoskeleton. This sheds light on the biological mechanism of action of this homeopathic preparation.