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Bioactivity Assessment of Functionalized TiO2 Powder with Dihydroquercetin.

Valentina Nikšić1, Andrea Pirković2, Biljana Spremo-Potparević3

  • 1Vinča Institute of Nuclear Sciences-National Institute of the Republic of Serbia, Centre of Excellence for Photoconversion, University of Belgrade, 11351 Belgrade, Serbia.

International Journal of Molecular Sciences
|February 26, 2025
PubMed
Summary

The TiO2-based ICT complex with dihydroquercetin (DHQ) shows reduced toxicity and enhanced antioxidant and antimicrobial properties. This novel material mitigates DNA damage and offers potential as a safe biocide agent.

Keywords:
TiO2antigenotoxicityantimicrobial activitycytotoxicitydihydroquercetininterfacial charge transfer complex

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Area of Science:

  • Materials Science
  • Biotechnology
  • Nanotechnology

Background:

  • Titanium dioxide (TiO2) is a widely used nanomaterial with photocatalytic properties.
  • Dihydroquercetin (DHQ) is a natural flavonoid with known antioxidant and anti-inflammatory effects.
  • Developing safe and effective nanomaterials for biomedical applications is a key research area.

Purpose of the Study:

  • To evaluate the biological activities of a visible-light-responsive TiO2-based ICT complex with DHQ.
  • To compare the safety and efficacy of TiO2/DHQ with pristine TiO2.
  • To explore the potential of TiO2/DHQ as a non-toxic biocide agent.

Main Methods:

  • In vitro assessment of cell viability using MRC-5 and HeLa cell lines.
  • Evaluation of reactive oxygen species (ROS) production.
  • Genotoxicity and antigenotoxicity testing via comet assay.
  • Antimicrobial activity testing against E. coli under visible light.

Main Results:

  • TiO2/DHQ showed reduced cell viability at higher concentrations compared to TiO2.
  • TiO2/DHQ significantly decreased ROS levels in both cell types.
  • TiO2/DHQ mitigated TiO2-induced DNA damage and offered protection against H2O2-induced damage.
  • TiO2/DHQ exhibited visible-light-activated antimicrobial activity against E. coli, unlike pristine TiO2.

Conclusions:

  • The TiO2-based ICT complex with DHQ demonstrates enhanced antioxidant and antigenotoxic properties.
  • DHQ mitigates the genotoxic potential of TiO2, improving its safety profile.
  • TiO2/DHQ shows promise as a safe and effective non-toxic biocide agent activated by visible light.