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Membrane Fluidity01:23

Membrane Fluidity

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Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.
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Yeast Viability in HLD-NAC-Designed Fully Dilutable Lecithin-Linker Microemulsions.

Juan Doratt Mendoza1, Jingwen Ding1, Michelle Acosta Alvarez1

  • 1Department of Chemical Engineering and Applied Chemistry, Faculty of Engineering and Applied Science, University of Toronto, Toronto, ON M5S 3E5, Canada.

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Summary
This summary is machine-generated.

This study introduces lecithin-linker microemulsions (LLMs) for cell preservation. These systems maintain yeast viability for 10 weeks by balancing water content and nutrient delivery, overcoming previous limitations.

Keywords:
HLD–NACbiologicslecithinlinkersmicroemulsionyeast

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Area of Science:

  • Biochemistry
  • Materials Science
  • Cell Biology

Background:

  • Previous attempts at cell preservation using microemulsions (µEs) in the 1990s were limited by biocompatibility issues and short cell survival times (≤3 days).
  • Developing stable and effective microemulsion formulations for long-term cell viability remains a challenge.

Purpose of the Study:

  • To explore the efficacy of fully dilutable self-microemulsifying delivery systems (SMEDS) as a novel cell preservation medium.
  • To formulate and characterize lecithin-linker microemulsions (LLMs) using specific surfactant blends and evaluate their impact on yeast viability.

Main Methods:

  • Formulation of SMEDS using lecithin (Le) and polyglycerol-10-caprylate (PG10C) at a 2/5 ratio, mixed with ethyl oleate (EOL) to create a D60 dilution line.
  • Dilution of D60 SMEDS with 0.9% NaCl to form LLMs, characterized using hydrophilic-lipophilic-difference (HLD) and net-average curvature (NAC) models.
  • Assessment of LLM properties (bicontinuity, viscosity, conductivity) and yeast activity/viability at varying aqueous contents.

Main Results:

  • LLMs with 5%-60% aqueous content were predicted to be bicontinuous, confirmed by viscosity and conductivity measurements.
  • Optimal yeast activity and viability were observed at 30% aqueous content, balancing metabolite transport and nutrient supply.
  • Low water activity in SMEDS induced yeast dormancy, ensuring survival for at least 10 weeks.

Conclusions:

  • Lecithin-linker microemulsions (LLMs) offer a promising solution for long-term cell preservation, extending viability to at least 10 weeks.
  • The formulation achieved through SMEDS balances essential factors for cell survival, including nutrient delivery and controlled dormancy.
  • This approach overcomes historical limitations in microemulsion-based cell preservation, paving the way for future applications.