Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Medium Optimization01:28

Methods of Medium Optimization

Optimizing growth media enhances microbial proliferation and maximizes product yield. Statistical experimental design methodologies provide structured and reproducible approaches, offering progressively higher levels of robustness and efficiency.The One-Factor-at-a-Time (OFAT) MethodThe One-Factor-at-a-Time (OFAT) method involves adjusting a single variable while keeping all others constant. However, it cannot detect interactions between variables, often leading to suboptimal outcomes when...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell cycle regulation has shaped replication origins in budding yeast.

Nature structural & molecular biology·2025
Same author

Enterovirus-like particles encapsidate RNA and exhibit decreased stability due to lack of maturation.

PLoS pathogens·2025
Same author

Automated cell counting for Trypan blue-stained cell cultures using machine learning.

PloS one·2023
Same author

A chromatinized origin reduces the mobility of ORC and MCM through interactions and spatial constraint.

Nature communications·2023
Same author

Nucleotide binding halts diffusion of the eukaryotic replicative helicase during activation.

Nature communications·2023
Same author

Construction of a Vero Cell Line Expressing Human ICAM1 for the Development of Rhinovirus Vaccines.

Viruses·2022

Related Experiment Video

Updated: Jun 19, 2026

Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems
13:47

Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems

Published on: April 23, 2012

10.9K

Optimization of Enterovirus-like Particle Production and Purification Using Design of Experiments.

Louis Kuijpers1,2, Wouter J P van den Braak2, Abbas Freydoonian2

  • 1Department of Bionanoscience, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands.

Pathogens (Basel, Switzerland)
|February 26, 2025
PubMed
Summary

This study optimized virus-like particle (VLP) production for Hand, Foot, and Mouth Disease (HFMD) vaccines. Optimized conditions yielded significant amounts of CVA6 and EV71 VLPs, showing economic potential for vaccine development.

Keywords:
and mouth diseasecoxsackievirus A6design of experimentsenterovirus A71foothandoptimization

More Related Videos

Purification of High Yield Extracellular Vesicle Preparations Away from Virus
00:07

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Published on: September 12, 2019

11.3K
An Efficient Method for Adenovirus Production
10:06

An Efficient Method for Adenovirus Production

Published on: June 10, 2021

13.0K

Related Experiment Videos

Last Updated: Jun 19, 2026

Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems
13:47

Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems

Published on: April 23, 2012

10.9K
Purification of High Yield Extracellular Vesicle Preparations Away from Virus
00:07

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Published on: September 12, 2019

11.3K
An Efficient Method for Adenovirus Production
10:06

An Efficient Method for Adenovirus Production

Published on: June 10, 2021

13.0K

Area of Science:

  • Virology
  • Vaccine Technology
  • Bioprocessing

Background:

  • Hand, Foot, and Mouth Disease (HFMD) is an emerging public health concern caused by Coxsackievirus A6 (CVA6) and Enterovirus A71 (EV71).
  • Current vaccine development for CVA6 is lacking, and emerging strains necessitate novel vaccine strategies like virus-like particles (VLPs).
  • Previous enterovirus VLP production studies often overlook optimizing key infection parameters.

Purpose of the Study:

  • To optimize the production of CVA6 and EV71 virus-like particles (VLPs) using a Design of Experiments (DoE) approach.
  • To identify optimal conditions for VLP yield by analyzing the interplay of multiplicity of infection (MOI), viable cell density (VCD), and infection period.
  • To assess the scalability and economic viability of VLP production for potential HFMD vaccines.

Main Methods:

  • Design of Experiments (DoE) was employed to systematically investigate and optimize VLP production parameters.
  • Expression of viral P1 structural proteins and 3CD protease was utilized for VLP generation.
  • Downstream purification processes were implemented to isolate and quantify the produced VLPs.

Main Results:

  • CVA6 VLP production was found to be maximal under conditions of high MOI, high VCD, and extended infection periods.
  • Purification yielded 38 mg of CVA6 VLPs and 158 mg of EV71 VLPs per liter of crude harvest.
  • The substantial yields indicate a strong potential for cost-effective, large-scale VLP vaccine production.

Conclusions:

  • Optimized bioprocessing parameters significantly enhance enterovirus VLP yield.
  • High-yield VLP production presents a promising and economically viable strategy for developing vaccines against HFMD.
  • This work provides a foundation for the development of effective VLP-based vaccines against CVA6 and EV71.