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Updated: May 25, 2025

Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks
Published on: September 20, 2021
Sulfide-Responsive Transcription Control in Escherichia coli
Koichi Hori1, Rajalakshmi Balasubramanian1, Shinji Masuda1,2
1School of Life Science & Technology, Tokyo Institute of Technology, Yokohama 226-8501, Japan.
Abstract:
To elucidate the mechanism of large-scale transcriptional changes dependent on sulfide in Escherichia coli, a large-scale RNA-sequencing analysis was performed on wild-type and sulfide-responsive transcription factor YgaV deletion mutants grown under three conditions: aerobic, semi-aerobic, and semi-aerobic with sulfide. The resulting dataset from these six conditions was subjected to principal component analysis, which categorized the data into five principal components. Estimation of the typical gene expression regulatory mechanisms in each category suggested the presence of mechanisms that are dependent on sulfide but independent of YgaV, as well as those that depend on YgaV but not on sulfide. In YgaV-dependent transcriptional regulation, YgaV was found to function as both a repressor and an activator. These results support the previous hypothesis that YgaV acts as a global regulator responsible for redox homeostasis.
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