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An Insight into the Presence of Antimicrobial Resistance Genes in Opportunistic Pathogenic Bacteria Isolated from

Teresita D J Bello Gonzalez1, Betty van Gelderen2, Frank Harders3

  • 1Department of Bacteriology, Host-Pathogen Interaction and Diagnostic Development, Antimicrobial Resistance Group, Wageningen Bioveterinary Research, Wageningen University Research, P.O. Box 65, 8200 AB Lelystad, The Netherlands.

Microorganisms
|February 26, 2025
PubMed

Insights

Antimicrobial resistance genes were found in bacteria from farm-reared crickets. This highlights the need for microbial safety control during insect rearing for sustainable feed and food production.

Area of Science:

  • Food Science
  • Microbiology
  • Insect Farming

Background:

  • Insects are explored as sustainable feed and food sources.
  • Evaluating microbiological risks, including antimicrobial resistance (AMR), is crucial for insect safety.
  • Opportunistic pathogenic bacteria can be present in farm-reared insects.

Purpose of the Study:

  • To investigate the presence of AMR genes in bacteria isolated from clinically healthy farm-reared crickets.
  • To assess the microbiological safety of insects during the rearing process.
  • To identify specific AMR genes and mobile genetic elements in bacteria from crickets.

Main Methods:

  • Whole-genome sequencing of 14 bacterial strains from house crickets (Acheta domesticus) and banded crickets (Gryllodes sigillatus).
  • Isolation of bacteria belonging to Enterobacteriaceae, Staphylococcaceae, Enterococcaceae, and Bacillaceae families.
  • Identification of specific AMR genes (e.g., beta-lactam, fosfomycin resistance) and mobile genetic elements (plasmids).

Main Results:

  • Predominant beta-lactam AMR genes (blaOXY2-6, blaACT-16, blaSHV variants) were found, mainly in Enterobacteriaceae.
  • Fosfomycin resistance (fosA) and oqxAB efflux pump complexes were associated with beta-lactam genes.
  • blaZ and mecA genes were detected in Bacillus cereus and Mammaliicoccus sciuri.
  • Various plasmids were identified in Klebsiella pneumoniae, Enterobacter hormaechei, Staphylococcus arlettae, and B. cereus.

Conclusions:

  • The study identified AMR genes and mobile genetic elements in bacteria from healthy farm-reared crickets.
  • Microbial safety control during insect rearing is essential to mitigate risks in the feed and food chain.
  • Continuous monitoring for pathogenic bacteria and AMR genes is necessary for safe insect-based products.
Keywords:
antibiotic resistancecricketsculturable bacteriaedible insectsmicrobial safetywhole genome sequencing

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