Comparative Analysis of Floral Transcriptomes in Gossypium hirsutum (Malvaceae)

Organ-specific transcriptomes provide valuable insight into the genes involved in organ identity and developmental control. This study investigated transcriptomes of floral organs and subtending bracts in wild and domesticated Gossypium hirsutum, focusing on MADS-box genes critical for floral development. The expression profiles of A, B, C, D, and E class genes were analyzed, confirming their roles in floral organ differentiation. Hierarchical clustering revealed similar expression patterns between bracts and sepals, as well as between petals and stamens, while carpels clustered with developing cotton fibers, reflecting their shared characteristics. Beyond MADS-box genes, other transcription factors were analyzed to explore the genetic basis of floral development. While wild and domesticated cotton showed similar expression patterns for key genes, domesticated cotton exhibited significantly higher expression in carpels compared to wild cotton, which aligns with the increased number of ovules in the carpels of domesticated cotton. Functional enrichment analysis highlighted organ-specific roles: genes upregulated in bracts were enriched for photosynthesis-related GO terms, while diverse functions were enriched in floral organs, supporting their respective functions. Notably, A class genes were not significantly expressed in petals, deviating from the ABCDE model, which warrants further analysis. Lastly, the ABCDE class genes exhibited differential homoeolog expression bias toward each subgenome between two accessions, suggesting that the domestication process has influenced homoeolog utilization despite functional constraints in floral organogenesis.