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A Simple and Scalable Chopped-Thallus Transformation Method for Marchantia polymorpha.
Rituraj Batth1, Andisheh Poormassalehgoo1,2, Kritika Bhardwaj1
1Malopolska Centre of Biotechnology, Jagiellonian University, 30-387 Krakow, Poland.
Plants (Basel, Switzerland)
|February 26, 2025
Summary
A new chopped-thallus method enhances genetic modification in the liverwort Marchantia polymorpha. This scalable technique improves transformation efficiency for large-scale experiments and mutant screening.
Area of Science:
- Plant biology
- Genetics
- Evolutionary biology
Background:
- Marchantia polymorpha is a key model organism for plant biology and evolution.
- Agrobacterium-mediated transformation is crucial for genetic studies in M. polymorpha.
- Existing methods using spores, thalli, or gemmae have limitations in efficiency or scalability.
Purpose of the Study:
- To develop a novel, scalable method for efficient genetic transformation of Marchantia polymorpha.
- To overcome the limitations of existing transformation techniques for large-scale applications.
Main Methods:
- Developed a chopped-thallus transformation technique.
- Optimized regeneration duration for thallus fragments.
- Utilized a simplified Gamborg's B5 medium.
Main Results:
- The chopped-thallus method significantly improved transformation efficiency compared to traditional methods.
- Achieved sufficient numbers of genetically consistent transformants.
- Demonstrated scalability for high-throughput applications.
Conclusions:
- The novel chopped-thallus method provides a scalable and efficient approach for genetic modification of Marchantia polymorpha.
- This technique facilitates large-scale experiments, including mutant screening.
- The method simplifies genetic studies in this important model plant.
