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Updated: May 25, 2025
An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
Published on: May 21, 2019
RETRACTION: MiR-101-3p Regulates the Viability of Lung Squamous Carcinoma Cells via Targeting EZH2
Retraction:
Y. Hou, L. Li, Y. Ju, Y. Lu, L. Chang, and X. Xiang, "MiR-101-3p Regulates the Viability of Lung Squamous Carcinoma Cells via Targeting EZH2," Journal of Cellular Biochemistry 118, no. 10 (2017): 3142-3149, https://doi.org/10.1002/jcb.25836. The above article, published online on 14 December 2016 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors; the journal Editor-in-Chief, Christian Behl; and Wiley Periodicals LLC. The retraction has been agreed due to concerns raised by third parties on the data presented in the article. Specifically, duplication of multiple image elements within Figure 3 C and 3E has been identified. Additional flaws and inconsistencies between methodology described and results presented were found. The authors were not able to provide comprehensive raw data. Accordingly, the article is retracted as the editors have lost confidence in the integrity and reliability of the full body of data presented in the article and consider its conclusions invalid.
Insights
This study investigated microRNA-101-3p's role in lung squamous carcinoma cell viability by targeting EZH2. However, the article was retracted due to significant data integrity issues, including image duplication and inconsistencies.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Lung squamous carcinoma (LSC) is a major subtype of non-small cell lung cancer.
- MicroRNAs (miRNAs) play critical roles in cancer development and progression.
- EZH2 is a key epigenetic regulator implicated in various cancers.
Purpose of the Study:
- To investigate the role of microRNA-101-3p (miR-101-3p) in regulating the viability of lung squamous carcinoma cells.
- To determine if miR-101-3p targets Enhancer of Zeste Homolog 2 (EZH2) in LSC cells.
Main Methods:
- Cell viability assays were performed on LSC cell lines.
- Expression levels of miR-101-3p and EZH2 were analyzed.
- Bioinformatic analysis and luciferase reporter assays were used to predict and confirm EZH2 as a target of miR-101-3p.
Main Results:
- The study aimed to demonstrate that miR-101-3p inhibits LSC cell viability.
- Results were intended to show that miR-101-3p directly targets EZH2, leading to decreased cell viability.
- Specific findings regarding the regulatory relationship were presented in figures.
Conclusions:
- The article concluded that miR-101-3p functions as a tumor suppressor in LSC by targeting EZH2.
- This regulatory axis was proposed to impact LSC cell proliferation and survival.
- The findings suggested a potential therapeutic target for LSC treatment.
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