Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cell-matrix's Response to Mechanical Forces

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dorsal Root Ganglion as a Hub for Peripheral Sensitization: A Hierarchical Regulation Model and Translational Progress.

Journal of pain research·2026

Same author

Mechanical and biological enhancement of glass ionomer cement through nanofiller integration: a preliminary study for improved restorative applications.

Scientific reports·2026

Same author

Clinical features and surgical treatment of magnetic bead ingestion in children: a single-center retrospective study.

Frontiers in pediatrics·2026

Same author

CeO<sub>2</sub>-Loaded Shear-Thinning Hydrogel Combined with Dental Pulp Stem Cells Modulates the Oxidative-Inflammatory Microenvironment To Enhance Facial Nerve Regeneration.

ACS applied materials & interfaces·2026

Same author

A Fluorescence Imaging-Based 3D Analysis Pipeline for Mouse Trigeminal Ganglion Neurons.

Biosensors·2026

Same author

Coordination-Induced Dispersion of Covalent Organic Frameworks for Organic Solar Cells With 21.03% Efficiency.

Advanced materials (Deerfield Beach, Fla.)·2026

Search research articles

Related Experiment Video

Updated: Jul 13, 2026

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

Effects of simulated microgravity on dental pulp stem cell stemness.

Huailong Hou¹, Zhengjun Qiu¹, Jingyi Che¹

¹Department of Endodontics, The First Affiliated Hospital of Harbin Medical University, 143 Yiman Street, St Nangang Dist., Harbin, 150001, China.

Journal of Molecular Histology

|February 26, 2025

Summary

Simulated microgravity (SMG) enhances dental pulp stem cell (DPSC) stemness and proliferation. This method may activate the MAPK/ERK pathway, offering a new strategy for regenerative medicine.

Keywords:

Dental pulp stem cells MAPK/ERK signaling pathway Simulated microgravity Stemness

More Related Videos

Propagation of Dental and Respiratory Cells and Organs in Microgravity

Propagation of Dental and Respiratory Cells and Organs in Microgravity

Published on: May 25, 2021

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Published on: May 5, 2023

Related Experiment Videos

Last Updated: Jul 13, 2026

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

Propagation of Dental and Respiratory Cells and Organs in Microgravity

Propagation of Dental and Respiratory Cells and Organs in Microgravity

Published on: May 25, 2021

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Published on: May 5, 2023

Area of Science:

Biotechnology
Regenerative Medicine
Stem Cell Biology

Background:

Dental pulp stem cells (DPSCs) are promising for regenerative medicine but lose stemness during in vitro expansion.
Maintaining DPSC stemness is crucial for effective therapeutic applications.

Purpose of the Study:

To investigate the impact of simulated microgravity (SMG) on DPSC stemness and differentiation.
To explore the molecular mechanisms underlying SMG's effects on DPSCs.

Main Methods:

DPSCs were cultured under simulated microgravity (SMG) using a 3D clinostat for 3 days.
Assessed stemness, proliferation, senescence, differentiation potential, surface markers, and gene expression.
Transcriptome sequencing and MAPK/ERK pathway analysis were performed.

Main Results:

SMG significantly enhanced DPSC replicative activity, proliferation, self-renewal, and inhibited senescence.
DPSCs under SMG showed superior osteogenic, adipogenic, chondrogenic, and neural differentiation.
SMG increased expression of MSC markers (Stro-1, CD146) and stemness genes (Oct4, Nanog, Sox2).
Transcriptome analysis revealed involvement of the MAPK signaling pathway, with confirmed MAPK/ERK activation.

Conclusions:

SMG effectively maintains DPSC stemness in vitro.
The MAPK/ERK signaling pathway activation is a potential mechanism for SMG-induced stemness maintenance in DPSCs.
SMG offers a promising approach for improving DPSC-based regenerative therapies.