Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Zinc and Chlamydia trachomatis.

B Sugarman, L R Epps

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
    |July 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Re-engineering adenovirus regulatory pathways to enhance oncolytic specificity and efficacy.

    Nature biotechnology·2001
    Same author

    Comparison of flow cytometry and laser scanning cytometry for the intracellular evaluation of adenoviral infectivity and p53 protein expression in gene therapy.

    Cytometry·1998
    Same author

    Estrogens and infection.

    Reviews of infectious diseases·1991
    Same author

    Chemorepulsion of trichomonads by products of neutrophil oxidative metabolism.

    The Journal of infectious diseases·1991
    Same author

    Antipyresis and fever.

    Archives of internal medicine·1990
    Same author

    Oestrogen binding by and effect of oestrogen on trichomonads and bacteria.

    Journal of medical microbiology·1990
    Same journal

    Parasympathomimetic effect of aqueous humor in human eyes with and without chronic simple glaucoma.

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·2010
    Same journal

    Studies on mode of action of streptomycin.

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·2010
    Same journal

    Growth of influenza virus in eggs in the presence of bacterial contamination and streptomycin.

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·2010
    Same journal

    A convenient test animal for the detection of the leukocytosis-promoting factor of exudates.

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·2010
    Same journal

    Preparation of an inactivated tissue culture scrub typhus vaccine.

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·2010
    Same journal

    Studies on scrub typhus; preparation of formalinized vaccines from tissues of infected mice and rats.

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·2010
    See all related articles

    Supplemental zinc can increase Chlamydia trachomatis cell infection rates in cultures. However, higher zinc concentrations after incubation show a lethal effect on Chlamydia, impacting infection susceptibility.

    Area of Science:

    • Microbiology
    • Cell Biology
    • Infectious Diseases

    Background:

    • Chlamydia trachomatis is a significant human pathogen.
    • Understanding factors influencing Chlamydia trachomatis infection and culture is crucial for diagnosis and treatment.

    Purpose of the Study:

    • To investigate the effect of supplemental zinc on McCoy cell infection by Chlamydia trachomatis.
    • To determine if zinc influences Chlamydia trachomatis culture yield and viability.

    Main Methods:

    • McCoy cells were infected with Chlamydia trachomatis using inoculation media with varying supplemental zinc concentrations (up to 1 X 10(-4) M).
    • Control groups used standard Chlamydia cultivation media (8 X 10(-6) M zinc).
    • Effects of ferric chloride and calcium chloride were also assessed.

    Related Experiment Videos

  • Incubation with high zinc concentrations followed by repassage without zinc was performed to assess direct effects on Chlamydia.

    • Main Results:

    • Supplemental zinc (up to 1 X 10(-4) M) significantly increased the number of infected McCoy cells by up to 200% compared to standard media.
    • Ferric chloride and calcium chloride had no significant effect on infection rates.
    • Higher zinc concentrations (after 2 hr incubation) significantly decreased Chlamydia inclusions, indicating a lethal effect on the bacteria.

    Conclusions:

    • Supplemental zinc in inoculation media can enhance Chlamydia trachomatis culture yield.
    • Zinc exhibits a dual effect: promoting infection at lower supplemental concentrations and exhibiting bactericidal activity at higher concentrations.
    • Oral or topical zinc use may influence individual susceptibility to Chlamydia trachomatis infections.