Annexin A's Life in Pan-Cancer: Especially in Glioma Immune Cells

The Annexin A (ANXA) family plays a critical role in cancer, with particular emphasis on their prognostic significance in pan-cancer analyses and gliomas. By integrating multi-omics data from The Cancer Genome Atlas (TCGA) and single-cell sequencing analysis, we conducted a comprehensive evaluation of ANXA2 and ANXA4 to investigate their expression patterns and functional impacts across various cancers, with a focus on glioblastoma (GBM). Our analysis encompassed several key components, including literature review, identification of differentially expressed genes (DEGs) in cancer, survival analysis, co-expression studies, competing endogenous RNA networks, cellular functional analysis, tumor microenvironment response to chemotherapy, and tumor stemness. Special attention was given to glioblastoma and low-grade glioma. Notably, our findings highlighted discrepancies among the analytical tools used, underscoring the necessity of employing multiple methods for accurate identification of DEGs. Additionally, we determined that ANXA2 and ANXA4 are predominantly expressed by M2 macrophages in GBM, based on our characterization of human glioma macrophages. These results suggest a strong correlation between ANXA2 and ANXA4 expression levels and the presence of macrophages and CD4 + resting memory T cells in gliomas, offering valuable insights into the complex interplay between the ANXA family and cancer progression.