Mass-spectrometry-based proteomics: from single cells to clinical applications
Tiannan Guo1,2,3, Judith A Steen4,5, Matthias Mann6,7
1State Key Laboratory of Medical Proteomics, School of Medicine, Westlake University, Hangzhou, China. guotiannan@westlake.edu.cn.
Nature
|February 26, 2025
Summary
Mass-spectrometry (MS)-based proteomics is advancing rapidly, enhancing sensitivity for single-cell analysis and tissue profiling. These innovations are paving the way for new clinical diagnostics and a deeper understanding of biology.
Area of Science:
- Proteomics
- Biotechnology
- Analytical Chemistry
Background:
- Mass spectrometry (MS)-based proteomics is a key technology for biological system analysis.
- Recent advancements have significantly boosted MS sensitivity and throughput.
- These improvements are crucial for clinical applications and detailed biological insights.
Purpose of the Study:
- To review the latest developments in proteomics technology.
- To explore the impact of these advances on key research areas.
- To discuss the clinical translation and future potential of proteomics.
Main Methods:
- Novel sample-preparation techniques.
- Advanced instrumentation in mass spectrometry.
- Innovative data-acquisition strategies and AI integration.
Main Results:
- Enhanced sensitivity enabling single-cell proteomics and spatial tissue profiling.
- Increased throughput and robustness for clinical applications.
- Progress in studying protein-protein interactions, PTMs, and structural proteomics.
Conclusions:
- Proteomics technology is rapidly evolving, offering unprecedented biological insights.
- Artificial intelligence accelerates data analysis and interpretation in proteomics.
- Proteomics is transitioning to clinical practice for diagnostics and biomarker discovery.
More Related Videos
Related Concept Videos
01:19MALDI-TOF Mass Spectrometry
4.7K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
4.7K
01:33Proteomics
7.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.2K
01:33Peptide Identification Using Tandem Mass Spectrometry
6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K