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Chromatin Modification in iPS Cells

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Updated: May 25, 2025

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

Genome-coverage single-cell histone modifications for embryo lineage tracing.

Min Liu¹, Yanzhu Yue², Xubin Chen¹

¹Institute of Molecular Medicine and National Biomedical Imaging Center, College of Future Technology, Peking-Tsinghua Center for Life Sciences and State Key Laboratory of Gene Function and Modulation Research, Peking University, Beijing, China.

|February 26, 2025

Summary

This summary is machine-generated.

Researchers developed a new method to map epigenetic changes in single cells during early mouse development. This reveals how cells gain unique identities and form different tissues, advancing our understanding of developmental biology.

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Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

Published on: February 26, 2015

Area of Science:

Developmental Biology
Epigenetics
Genomics

Background:

Early embryonic development involves significant epigenetic reprogramming to establish cellular diversity.
Mapping single-cell epigenomic profiles is crucial for understanding developmental trajectories.

Purpose of the Study:

To develop a novel method for genome-wide single-cell profiling of histone modifications in early mouse embryos.
To create a high-resolution epigenetic landscape of early development by integrating multi-modal single-cell data.
To identify regulatory networks governing totipotency and early lineage specification.

Main Methods:

Developed target chromatin indexing and tagmentation (TACIT) for single-cell epigenomic profiling.
Performed genome-coverage single-cell profiling of seven histone modifications in mouse early embryos.
Integrated single-cell histone modification data with single-cell RNA sequencing data.

Main Results:

Mapped the single-cell epigenetic landscape of mouse pre-implantation development.
Identified early priming of totipotency heterogeneities at the zygotic genome activation stage.
Discovered totipotency gene regulatory networks, including transposable elements and transcription factors.
Developed a predictive model for cell lineage branching (inner cell mass and trophectoderm).

Conclusions:

TACIT enables comprehensive single-cell epigenomic profiling, advancing developmental biology research.
Early epigenetic reprogramming and multimodal regulation are key drivers of cell-fate determination.
Identified novel transcription factors and regulatory elements involved in lineage specification.