Related Experiment Video
Updated: May 25, 2025
Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
Published on: August 8, 2016
'Need for speed: high throughput' - mass spectrometry approaches for high-throughput directed evolution screening of
Robert A Shepherd1, Conrad A Fihn1, Alex J Tabag1,2
1Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, CA 95064, USA, +1 831-459-4676. lmsanche@ucsc.edu.
Abstract:
Covering: 2015 to 2024Mass spectrometry (MS)-based methods have been implemented extensively for enzyme engineering due to their label-free nature, making them suitable for screening a wide range of biochemical systems. Over the past decade, advancements in mass spectrometry, separation science, and the implementation of hyphenated methods have allowed for more streamlined analysis of large volumes of samples while maximizing the richness and dimensionality of the data collected. In this review we highlight recent advancements in mass spectrometry that have allowed for more efficient, robust, and rigorous enzyme engineering for various applications relating to natural products chemistry.
