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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: May 25, 2025

Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy

Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy

Published on: October 6, 2023

De-risking transformative microscopy technologies for broad adoption.

Jesse Aaron¹, Teng-Leong Chew¹

¹Advanced Imaging Center, Howard Hughes Medical Institute Janelia Research Campus, Ashburn, Virginia.

Journal of Microscopy

|February 27, 2025

Summary

This summary is machine-generated.

New optical microscopy technologies offer biological insights but face adoption hurdles. Open-access incubators can de-risk these innovations, accelerating scientific discovery.

Keywords:

microscopy development microscopy dissemination technology de-risking technology validation

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Last Updated: May 25, 2025

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Area of Science:

Life Sciences
Biotechnology
Optical Microscopy

Background:

Significant advancements in optical microscopy over the past 20 years.
Limited adoption of novel microscopy technologies within the life science community.
Challenges to adoption are not solely technical but involve various risks.

Purpose of the Study:

To identify and address the barriers hindering the widespread adoption of innovative microscopy technologies.
To propose a framework for de-risking and facilitating the integration of disruptive microscopy inventions.
To enable transformative scientific output through enhanced technology adoption.

Main Methods:

Analysis of adoption patterns for new microscopy technologies.
Identification of common risks associated with disruptive scientific tools.
Conceptualization of an open-access technology incubator model.
Development of a framework for technology shepherdin

Main Results:

Novel microscopy technologies often face significant adoption risks beyond technical utility.
Open-access technology incubators provide a viable model for mitigating these risks.
A structured framework can guide innovative microscopy techniques through the adoption process.

Conclusions:

Addressing adoption challenges is crucial for realizing the full potential of advanced microscopy.
Open-access incubators are key to de-risking and accelerating the integration of new technologies.
Implementing a strategic framework will foster transformative scientific advancements through microscopy.