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Tanfloc-Modified Titanium Surfaces: Optimizing Blood Coagulant Activity and Stem Cell Compatibility
Ramesh Singh1, Liszt Y C Madruga1, Aniruddha Savargaonkar2
1Department of Bioengineering, College of Engineering and Computing, George Mason University, Fairfax, Virginia 22030, United States.
Abstract:
This study explores the synergistic effects of combining titania nanotubes (TiNTs) with the biopolymer Tanfloc (TAN) to enhance the surface properties of TiNTs for biomedical applications. We investigated the interactions of blood components and human adipose-derived stem cells (ADSCs) with TiNT surfaces covalently functionalized with Tanfloc (TAN), an aminolyzed polyphenolic tannin derivative. The functionalized surfaces (TiNT-TAN) have great potential to control protein adsorption and platelet adhesion and activation. Fluorescence and scanning electron microscopy (SEM) were used to analyze platelet adherence and activation. The amphoteric nature and multiple functional groups on TAN can control blood protein adsorption, platelet adhesion, and activation. Further, the modified surface supports adipose-derived stem cell (ADSC) viability, attachment, and growth without any cytotoxic effect. The TAN conjugation significantly (****p < 0.0001) increased the proliferation rate of ADSCs compared to the TiNT surfaces.
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