Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

86.9K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
86.9K
Sanger Sequencing01:57

Sanger Sequencing

752.2K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
752.2K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

10.9K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
10.9K
RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Artificial intelligence-based ultrasound diagnosis of Hashimoto's thyroiditis: a systematic review and meta-analysis.

Thyroid research·2026
Same author

METTL3- and IGF2BP1-associated m6A regulation of FADS2 contributes to lipid droplet accumulation and malignant progression in non-small cell lung cancer.

Translational cancer research·2026
Same author

Prognostic analysis and beneficiary population exploration of subsequent treatment regimens after third-generation EGFR-TKIs failure in EGFR-mutated advanced non-small cell lung cancer: a retrospective cohort study.

Translational lung cancer research·2026
Same author

Retraction notice to "SNP alleviates mitochondrial homeostasis dysregulation-mediated developmental toxicity in diabetic zebrafish larvae" [Biomedicine & Pharmacotherapy 177 (2024) 117117].

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Neutrophils and neutrophil extracellular traps in ischaemia-reperfusion injury: pathophysiological roles and therapeutic potential.

Burns & trauma·2026
Same author

Development and validation of a scale for the psychological determinants of dietary management behavior in hemodialysis patients during dialysis.

PloS one·2026

Related Experiment Video

Updated: May 25, 2025

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

13.4K

Advances and Challenges in Solid-State Nanopores for DNA Sequencing.

Yunhao Zhou1,2, Xia Long1,2, Yongqi Zhang1,2

  • 1Hunan Institute of Advanced Sensing and Information Technology, Xiangtan University, Xiangtan 411105, P. R. China.

Langmuir : the ACS Journal of Surfaces and Colloids
|February 27, 2025
PubMed
Summary

Solid-state nanopore sequencing offers advanced DNA analysis for genetic disease diagnosis. Despite challenges like resolution and speed, ongoing innovations promise breakthroughs in life sciences and medicine.

More Related Videos

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

13.5K
Nanopore DNA Sequencing for Metagenomic Soil Analysis
07:33

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

30.3K

Related Experiment Videos

Last Updated: May 25, 2025

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

13.4K
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

13.5K
Nanopore DNA Sequencing for Metagenomic Soil Analysis
07:33

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

30.3K

Area of Science:

  • Nanotechnology
  • Molecular Biology
  • Genomics

Background:

  • Solid-state nanopore sensing is a key technology for single-molecule detection.
  • It shows great promise for DNA sequencing by analyzing signal variations as DNA passes through a nanopore.
  • This method offers high-throughput, real-time, and PCR-free DNA analysis with applications in genetic disease diagnosis.

Purpose of the Study:

  • To review advancements in solid-state nanopore DNA sequencing technology.
  • To discuss various nanopore materials, preparation techniques, and detection methods.
  • To identify challenges and potential solutions for the implementation and commercialization of this technology.

Main Methods:

  • Review of nanopore materials (silicon-based, 2D materials).
  • Examination of preparation techniques (TEM, FIB, CBD).
  • Elucidation of sequencing detection mechanisms (ion-current blockade, transverse-current, optical detection).

Main Results:

  • Advancements in nanopore types, preparation, and detection methods have been discussed.
  • Various materials and techniques for nanopore fabrication and DNA analysis are presented.
  • Challenges such as limited spatial resolution, rapid DNA translocation, and signal noise are identified.

Conclusions:

  • Proposed solutions include film thinning, surface charge adjustment, and optimized detection materials/structures.
  • Interdisciplinary integration and technological innovation are crucial for future breakthroughs.
  • Solid-state nanopore DNA sequencing is poised to transform life sciences research and medical diagnostics.