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TRIM44 facilitates aggressive behaviors in multiple myeloma through promoting ZEB1 deubiquitination

Hui Qi1, Jing Wang2, Lixia Cao3

  • 1Department of Hematology, Affiliated Hospital of Inner Mongolia Medical University, 1 Tongdao North Road, Huimin District, Hohhot, 010050, China.

Discover Oncology
|February 27, 2025
PubMed
Abstract

Insights

Tripartite motif-containing 44 (TRIM44) drives multiple myeloma (MM) progression by stabilizing Zinc Finger E-Box Binding Homeobox 1 (ZEB1). Inhibiting TRIM44 reduces MM cell viability, migration, and invasion, suggesting TRIM44 as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology

Background:

  • Tripartite motif-containing 44 (TRIM44) is implicated in various cancers.
  • Its role in multiple myeloma (MM) pathogenesis requires elucidation.

Purpose of the Study:

  • To investigate the role of TRIM44 in multiple myeloma (MM).
  • To explore the molecular mechanism by which TRIM44 influences MM development.

Main Methods:

  • TRIM44 expression analysis in MM tissues and cell lines using RT-qPCR.
  • Assessment of MM cell proliferation, migration, and invasion upon TRIM44 modulation.
  • In vivo studies using subcutaneous tumor xenograft models.
  • Investigation of TRIM44-ZEB1 protein interactions via immunoprecipitation and Western blotting.

Main Results:

  • TRIM44 was consistently overexpressed in MM tissues and cell lines.
  • Reduced TRIM44 expression significantly inhibited MM cell viability, migration, and invasion.
  • TRIM44 overexpression led to decreased ZEB1 ubiquitination, enhancing ZEB1 protein stability.

Conclusions:

  • TRIM44 functions as an oncogenic factor in multiple myeloma.
  • TRIM44 promotes MM oncogenesis by stabilizing ZEB1.
Keywords:
DeubiquitinationMultiple myelomaTRIM44ZEB1

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