Biosynthesis of nickel nanoparticles using Enterobacter cloacae: characterization and multifaceted applications

This study describes the biosynthesis of nickel nanoparticles (Ni NPs) using gram-negative bacterium, Enterobacter cloacae. The synthesized Ni NPs were annealed at 350 °C for 3 h and characterized using various techniques. Powder X-ray diffraction analysis revealed the formation of Ni NPs with an average crystallite size of 48.78 nm. UV-Vis spectroscopy exhibited an absorption peak at 394 nm, while FTIR confirmed a peak at 416 nm. Field emission scanning electron microscopy showed the Ni NPs as a mixture uniform distribution, characterized by almost uniform spherical or hexagonal shapes. The Ni NPs demonstrated moderate antibacterial activity but exhibited notably high biofilm activity against Pseudomonas aeruginosa species, suggesting their potential in combating biofilm-associated infections. Additionally, the biosynthesized Ni NPs showed promising larvicidal and Insecticidal activities. This study provides an eco-friendly and cost-effective method for synthesizing Ni NPs and highlights their multifaceted applications in healthcare and pest control.