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2,4-Epibrassinolide Regulates Polysaccharide Substance Biosynthesis after Field Application to Dendrobium officinale
Lanfei Lu1, Hui Ye1, Nan Fang1
1State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ministry of Agriculture and Rural Affairs Key Laboratory for Pesticide Residue Detection, Institute of Agro-Products Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P. R. China.
Abstract:
This study investigated the effects of 2,4-epibrassinolide (EBR) residues on Dendrobium officinale quality. The residues decreased from 0.164 to 0.238 mg/kg (2 h) to 0.014-0.071 mg/kg (35 d) in the stems with 0.02-0.05 mg/L EBR applications. Polysaccharide, mannose, and glucose content were increased in the stems and leaves, reaching a maximum of 61% in dried stems on 35 d. The increase in content may result from the significant upregulation of enzyme activities, including UGP, SUS, and SPS. Further analysis by nontargeted metabolomics revealed 5 upregulated (UDP-l-rhamnose, mannose-6-phosphate, GDP-mannose, chitobiose, and N-acetyl-galactosamine-6-phosphate) and 13 downregulated metabolites associated with polysaccharide and monosaccharide growth in the 0.02-0.05 mg/L EBR treatments. These differential metabolites regulate the biosynthesis of polysaccharides mainly through key metabolic pathways, such as glucose and fructose metabolism. These results provide a reference for the regulatory mechanisms governing the quality indicators of Dendrobium officinale after EBR application.
