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Updated: May 25, 2025
DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
Published on: September 27, 2019
Functional metal organic framework mediated G-quadruplex DNA nanostructures for improved self-powered
Peiyuan Li1, Yashu Wei2, Shiyu Liu1
1Education Department of Guangxi Zhuang Autonomous Region, Laboratory of Optic-electric Chemo/Biosensing and Molecular Recognition, Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Key Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission, School of Chemistry and Chemical Engineering, Guangxi Minzu University, Nanning, 530006, China.
Abstract:
Accurate detection of major disease biomarkers is frequently hindered by complex environmental factors and their inherently low expression levels. To overcome these challenges, a self-powered, smartphone-assisted dual-mode biosensing platform has been developed, utilizing functional metal-organic frameworks (MOFs) and G-quadruplex (G-4s) DNA nanostructures to significantly amplify signal output and enhance detection accuracy. This innovative system utilizes gold nanoparticle/zirconium-based MOF/graphdiyne composites (AuNPs/Zr-MOF/GDY) and self-assembles methylene blue (MB) with G-quadruplex (G-4s) DNA nanostructures for detecting the thalassemia gene CD122. Upon target detection, the G-4s structure triggers strand displacement and cation coordination, followed by MB adsorption to form a biocathode. This biocathode facilitates electron transfer to a glucose oxidase-functionalized anode, driving redox reactions with system-generated electrons. The MB/G-4s structure not only amplifies the CD122 signal but also induces proportional color changes for dual electrochemical and colorimetric detection. A high-output-power capacitor is integrated to further amplify signals, enhancing sensitivity by 7.2 times. The platform offers a broad linear range from 0.0001 to 1000 pM and achieves low detection limits of 18.9 aM (electrochemical) and 23.9 aM (colorimetric) (S/N = 3). This work presents a highly accurate, sensitive, and portable detection platform for rapid analysis in various environments, including remote and challenging settings.
