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Research note: Improving post-thaw rooster sperm quality with Eurycoma longifolia extract and cyclodextrin
Thirawat Koedkanmark1, Khomsan Buathalad1, Supakorn Authaida2
1Department of Animal Science, Faculty of Agricultural, Khon Kaen University, Khon Kaen 40002, Thailand.
Poultry Science
|February 27, 2025
Summary
Eurycoma longifolia extract and its cyclodextrin complex improve rooster sperm cryopreservation. These natural compounds enhance sperm motility, viability, and fertility after thawing, offering potential for breeding programs.
Area of Science:
- Animal Reproduction
- Biotechnology
- Phytochemistry
Background:
- Cryopreservation of rooster semen is crucial for genetic conservation and breeding.
- Current cryopreservation methods lead to significant loss of sperm viability.
- Oxidative stress, indicated by MDA levels, is a major factor in sperm damage during cryopreservation.
Purpose of the Study:
- To evaluate the efficacy of Eurycoma longifolia (EL) extract and its cyclodextrin complex (ELC) in improving rooster sperm cryopreservation.
- To assess the impact of EL and ELC on post-thaw sperm motility, viability, and fertility.
- To determine the effect of EL and ELC on lipid peroxidation (MDA levels) in cryopreserved rooster sperm.
Main Methods:
- Rooster semen was cryopreserved with supplementation of EL extract (4 mg/mL) or ELC (2 mg/mL).
- Post-thaw sperm parameters including total motility, progressive motility, and viability were assessed.
- Malondialdehyde (MDA) levels were measured to evaluate lipid peroxidation.
- Fertility trials were conducted to assess the reproductive efficiency of cryopreserved semen.
Main Results:
- EL extract (4 mg/mL) significantly improved total motility (62.84%), progressive motility (32.70%), viability (55.90%), and reduced MDA levels (2.98 nmol/mL).
- ELC (2 mg/mL) showed comparable or superior results, enhancing total motility (59.21%), progressive motility (38.46%), viability (58.11%), and lowering MDA (2.60 nmol/mL).
- Both EL and ELC significantly increased fertility rates (55.38% and 52.19%, respectively).
Conclusions:
- Eurycoma longifolia extract and its cyclodextrin complex show significant potential for enhancing rooster sperm cryopreservation.
- Supplementation with EL and ELC can mitigate cryodamage, improving sperm quality and fertility outcomes.
- These findings suggest a promising natural-based approach to optimize semen cryopreservation protocols in poultry breeding programs.
