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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: May 25, 2025

Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages

Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages

Published on: June 2, 2020

Developmental biophysics

Thorsten Wohland¹, Timothy E Saunders², Chii Jou Chan³

¹Department of Biological Sciences, National University of Singapore, Singapore, Republic of Singapore; Department of Chemistry, National University of Singapore, Singapore, Republic of Singapore.

Biophysical Journal

|February 27, 2025

Summary

No abstract available in PubMed .

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