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Intralumenal Vesicles and Multivesicular Bodies

Intralumenal Vesicles and Multivesicular Bodies

Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...

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Extraction of Extracellular Vesicles from Whole Tissue

Extraction of Extracellular Vesicles from Whole Tissue

Published on: February 7, 2019

Direct Extraction and Evaluation of Intraluminal Vesicles Inside a Single Cell.

Hiroki Ida^1,2,3, Takeshi Yoshida⁴, Akichika Kumatani^3,5,6

¹Department of Electrical Engineering, Graduate School of Engineering, Nagoya University, Aichi 464-8601, Japan.

|February 27, 2025

Summary

Researchers developed a new method to directly extract and analyze intraluminal vesicles (ILVs) within living cells. This technique reveals distinct RNA profiles of ILVs, offering new insights into cellular vesicle contents.

Keywords:

Extracellular vesicle Intraluminal vesicle Nanobiopsy Nanopipette SICM miRNA

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Last Updated: Jun 8, 2026

Extraction of Extracellular Vesicles from Whole Tissue

Extraction of Extracellular Vesicles from Whole Tissue

Published on: February 7, 2019

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Published on: February 14, 2022

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

Published on: August 26, 2021

Area of Science:

Cell Biology
Biotechnology
Microscopy

Background:

Endogenous extracellular vesicles play crucial roles in physiological functions.
Current methods for evaluating vesicles within cells are limited.
A need exists for techniques to directly assess intraluminal vesicle (ILV) contents.

Purpose of the Study:

To present a novel technique for the direct extraction and evaluation of intraluminal vesicles (ILVs) from living cells.
To analyze the molecular contents, specifically RNA expression profiles, of ILVs.
To enable direct assessment of miRNA localization within cells.

Main Methods:

Combined scanning ion conductance microscopy, electrochemical syringes, and confocal microscopy for high-resolution, femtoliter-scale extraction of intracellular structures.
Directly collected CD63(+) vesicles from HEK293 CD63-pHluorin-RFP cells.
Compared RNA expression profiles of ILVs with cytosol and ultracentrifuge-isolated extracellular vesicles.

Main Results:

Successfully extracted and evaluated intraluminal vesicles (ILVs) directly from living cells.
ILVs exhibited distinct RNA expression profiles compared to cytosol and extracellular vesicles.
Identified a subset of ILVs containing hsa-miR-145-5p, enabling direct assessment of miRNA accumulation.

Conclusions:

The developed technique allows for direct extraction and analysis of intraluminal vesicle (ILV) contents within living cells.
This method reveals unique molecular signatures of ILVs, differing from extracellular vesicles.
This approach is a powerful new tool for studying ILV composition and function, particularly miRNA localization.