Related Experiment Video
Updated: May 10, 2026

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
Published on: December 28, 2017
Aspergillus fumigatus mitogenomes and their influence on azole-resistant and -susceptible populations
Endrews Delbaje1, Laís Pontes1, Johanna Rhodes2
1Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil.
Abstract:
The role of the fungal mitochondria goes far beyond energy metabolism. The genomes of 318 Aspergillus fumigatus clinical and environmental isolates from different geographic origins were analyzed aiming to study the mitochondrial sequences from populations sensitive and resistant to azoles. Our results show that A. fumigatus mitogenomic sequences are very conserved and only show variation in small intergenic regions and one intronic sequence in the cox3 gene. Furthermore, a genome-wide association analysis of accessory mitochondrial genes revealed potential mitochondria-based genotypes that may interact synergistically with the ergosterol biosynthesis pathway to confer the resistant phenotype. This includes a mutation in the AMID-like mitochondrial oxidoreductase (aifA, AFUA_3G01290) and the absence of the mitochondrial carrier protein (pet8, AFUA_8G01400). Deletion of these genes did not change the azole-susceptibility but increased the azole-persistence, suggesting mitochondrial genes could be involved in azole-persistence. Our work opens new hypotheses for the involvement of mitochondria in A. fumigatus azole-resistance.
More Related Videos
09:21Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.
Published on: February 15, 2019
10:33Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
Published on: May 27, 2022
Related Concept Videos
Gene Regulation in Microbial Communities: Quorum Sensing
Development of Antibiotic Resistance
Fungal Phylum Ascomycota
Production of Pharmaceuticals
Inhibitors of Bacterial Protein Synthesis
Antifungal Agents