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circZNF707 promoted glycolysis and tumor progression through miR-668-3p-PFKM axis in NSCLC

Wei Chen1,2, Shuai Fang1, Xianqiao Wu1,2

  • 1Thoracic Surgery Department, The First Affiliated Hospital of Ningbo University, No.247 Renmin Road, Ningbo, 315020, Zhejiang Province, People's Republic of China.

European Journal of Medical Research
|February 27, 2025
PubMed
Abstract

Insights

Circular RNA ZNF707 (circZNF707) promotes non-small cell lung cancer (NSCLC) progression by enhancing cell proliferation, migration, and glycolysis. This circRNA may offer new diagnostic and therapeutic strategies for NSCLC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in human cancer development.
  • The specific roles and mechanisms of circRNAs in non-small cell lung cancer (NSCLC) are not well understood.

Purpose of the Study:

  • To investigate the role and mechanism of circZNF707 in non-small cell lung cancer (NSCLC).

Main Methods:

  • Microarray analysis identified differentially expressed circRNAs in NSCLC tissues.
  • Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) measured circZNF707 expression.
  • Loss-of-function and gain-of-function assays assessed NSCLC cell behavior.
  • Western blotting, RIP, qRT-PCR, and luciferase assays elucidated the molecular mechanism.

Main Results:

  • circZNF707 expression was significantly upregulated in NSCLC tissues.
  • circZNF707 promoted NSCLC cell proliferation, migration, invasion, and glycolysis.
  • circZNF707 acts as a molecular sponge for miR-668-3p, upregulating PFKM and driving NSCLC progression.

Conclusions:

  • The circZNF707/miR-668-3p/PFKM axis promotes NSCLC tumor development.
  • circZNF707 presents potential as a biomarker for NSCLC diagnosis and a therapeutic target.
Keywords:
CircRNAGlycolysisMiR-668-3pNSCLCPFKM

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