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Updated: May 25, 2025

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development
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BIN1 inhibited tumor growth, metastasis and stemness by ALDH1/NOTCH pathway in bladder carcinoma

Si-Yu Chen1,2, Ya-Long Zhang1,2, Xiao-Ran Li1,2

  • 1Department of Urology, The Second Hospital of Lanzhou University, Lanzhou, China.

Hereditas
|February 27, 2025
PubMed
Abstract

Insights

Bridging integrator 1 (BIN1) acts as a tumor suppressor in bladder cancer (BLCA), inhibiting proliferation and metastasis. BIN1 downregulation is observed in BLCA, suggesting its potential as a diagnostic biomarker and therapeutic target.

Area of Science:

  • Urology
  • Oncology
  • Molecular Biology

Background:

  • Bladder cancer (BLCA) is a prevalent urological malignancy.
  • Bridging integrator 1 (BIN1) is a known tumor suppressor with poorly understood roles in BLCA.
  • This study investigates BIN1's function in BLCA development and progression.

Purpose of the Study:

  • To elucidate the role of BIN1 in regulating BLCA cell proliferation, metastasis, and cancer stem cell properties.
  • To identify BIN1 as a potential diagnostic biomarker and therapeutic target for BLCA.

Main Methods:

  • Urinary proteomics and public database analysis identified BIN1 dysregulation in BLCA.
  • Proteomic profiling revealed ALDH1 as a downstream effector in the BIN1-regulated ALDH1/NOTCH signaling axis.
  • Experimental validation included Western blot, RT-PCR, immunofluorescence, migration, proliferation, and cell transfection assays.

Main Results:

  • BIN1 was significantly downregulated in BLCA tissues and cell lines.
  • BIN1 overexpression inhibited proliferation, promoted apoptosis, and suppressed epithelial-mesenchymal transition (EMT), reducing metastasis.
  • BIN1 modulated cancer stem cell properties via ALDH1 and NOTCH2 signaling.

Conclusions:

  • BIN1 functions as a tumor suppressor in bladder cancer.
  • BIN1 holds potential as a diagnostic biomarker and therapeutic target for BLCA treatment.
Keywords:
ALDH1BIN1Bladder cancerCancer stem cellsNOTCH, EMT

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