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TIP2-UDT1-OsUPEX1/2 module regulates tapetum development and function in rice.

Ruifeng Wang1, Yaqian Sun1, Wanlin Liu1

  • 1Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 20040, China.

The New Phytologist
|February 28, 2025
PubMed
Summary
This summary is machine-generated.

Rice anther development relies on the tapetum, regulated by transcription factors UDT1 and TIP2. These factors form a complex, activating downstream genes crucial for pollen fertility.

Keywords:
antherbHLH transcription factorcallosegalactosyltransferasericetapetum

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Area of Science:

  • Plant reproductive biology
  • Molecular genetics
  • Agricultural science

Background:

  • The tapetum, a vital anther wall layer, is crucial for plant fertility and pollen development.
  • Tapetal development is orchestrated by transcription factors and signaling pathways, with UDT1, TIP2, TDR, and EAT1 forming a known cascade in rice.

Purpose of the Study:

  • To elucidate the largely unknown UDT1- and TIP2-dependent regulatory networks in early rice anther development.
  • To identify downstream targets of TIP2 and UDT1 essential for tapetum function and male fertility.

Main Methods:

  • Functional analysis using knockout mutants.
  • Spatial-temporal expression analysis of key genes.
  • Investigation of protein-protein interactions (heterodimerization).

Main Results:

  • TIP2 and UDT1 exhibit overlapping expression in the middle layer and tapetum, with TIP2 acting epistatically to UDT1.
  • TIP2 and UDT1 heterodimerize to activate downstream genes, including OsUPEX1 and OsUPEX2, which encode galactosyltransferases.
  • OsUPEX1 and OsUPEX2 are functionally redundant and critical for tapetum development, function, and secretion.

Conclusions:

  • TIP2 and UDT1 play essential roles in early rice anther development through heterodimerization and activation of downstream targets.
  • OsUPEX1 and OsUPEX2 are identified as key downstream effectors of the TIP2-UDT1 pathway, vital for tapetum function and rice male fertility.