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Elucidating prognostic significance of purine metabolism in colorectal cancer through integrating data from transcriptomic, immunohistochemical, and single-cell RNA sequencing analysis

  • 0Department of Genome Medicine and Science, Gachon Institute of Genome Medicine and Science, Gachon University Gil Medical Center, Gachon University College of Medicine, Gachon University, Incheon, Korea.
Molecular Oncology +

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February 28, 2025

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February 28, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

Low expression of key purine metabolism genes indicates a worse prognosis in colorectal cancer (CRC). These findings suggest purine metabolism alterations can serve as a novel prognostic marker for CRC patients.

Area Of Science

  • Oncology
  • Molecular Biology
  • Biochemistry

Background

  • Colorectal cancer (CRC) presents high mortality rates, with purine metabolism as a potential therapeutic target.
  • Purine metabolism's prognostic value in CRC lacks validation via immunohistochemical analysis.

Purpose Of The Study

  • To assess the clinical relevance of purine metabolism in colorectal cancer using integrated multi-omics approaches.
  • To validate purine metabolism genes as prognostic markers in CRC through immunohistochemistry.

Main Methods

  • Bulk transcriptome analysis and single-cell RNA sequencing (scRNA-seq) were employed.
  • Immunohistochemistry (IHC) was used to validate gene expression and prognostic significance.
  • Analysis included CRC patient subgroups (TP53 wild-type/mutant, microsatellite-stable).

Main Results

  • Low expression of ADSL, APRT, ADCY3, NME3, and NME6 correlated with worse prognosis in CRC subgroups.
  • NME3 expression was an independent prognostic factor; ADSL and NME6 predicted prognosis based on clinical variables.
  • NME3 predicted high risk in early-stage CRC, while ADSL and NME6 were predictive in late-stage CRC.

Conclusions

  • Alterations in purine metabolism show consistent directional protein expression in CRC tissues.
  • Five purine metabolism-related genes (ADSL, APRT, ADCY3, NME3, NME6) demonstrate prognostic potential in CRC.
  • Purine metabolism alterations represent a clinically useful prognostic marker for colorectal cancer.

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