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[Serological and Molecular Biological Characteristics of cisAB Blood Group and Transfusion Strategies]
Si-Meng Wu1, Qiao-Ni Yang1, Wa Gao1
1Department of Blood Transfusion，Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China.
Objective:
To analyze the serological and molecular biological characteristics of 5 patients with cis AB blood group, and to explore the safe transfusion strategy.
Methods:
Serological identification of the samples' blood group was performed using anti-A, anti-B, anti-D, anti-A1, anti-H typing reagents and ABO reagent erythrocytes. Molecular biological identification of the samples' blood group was performed using PCR-SSP or gene sequencing.
Results:
The serological identification results of blood group in 5 patients all showed inconsistent forward and reverse typing, presenting as A2B3 or A2Bw. ABO gene sequencing of samples 1, 2 and 3 showed 261delG in exon 6 and 467C>T, 803G>C in exon 7. The genotypes of samples 1, 2 and 3 were determined to be cisAB/O . PCR-SSP genotyping was performed on sample 4 and 5，and the results were both cisAB/O .
Conclusion:
Patients with cisAB alleles have inconsistent serological manifestations, and genetic testing is necessary to ensure the safety and effectiveness of blood transfusion.
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