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Decoding the Molecular Landscape of Prepubertal Oocyte Maturation: GTPBP4 as a Key Driver of In Vitro Developmental
Jianpeng Qin1, Yaozong Wei1, Ao Ning1
1State Key Laboratory of Swine and Poultry Breeding Industry, Key Laboratory of Livestock and Poultry Multiomics, Ministry of Agriculture and Rural Affairs, Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University Sichuan, Chengdu, China.
Understanding oocyte maturation in goats reveals key genes like GTPBP4 that improve prepubertal oocyte development, offering insights for human fertility preservation and animal breeding.
Area of Science:
- Reproductive Biology
- Molecular Genetics
- Translational Medicine
Background:
- Oocyte maturation mechanisms are poorly understood, particularly in domestic animals and for clinical applications in prepubertal girls.
- Ovarian tissue cryopreservation for fertility preservation in prepubertal girls requires scientific validation for developmental potential.
Purpose of the Study:
- To comprehensively profile transcriptional and translational dynamics during oocyte maturation in adult and prepubertal goats.
- To identify critical regulatory factors for prepubertal oocyte maturation and validate their function.
- To provide insights for improving fertility preservation strategies in humans and reproductive efficiency in domestic animals.
Main Methods:
- Transcriptome and translatome sequencing of adult and prepubertal goat oocytes.
- Comparative gene expression analysis.
- In vitro maturation experiments to validate gene function (GTPBP4).
Main Results:
- Identified a sequential shift in gene expression regulation from cytoplasmic processes to chromosome segregation during maturation.
- Discovered key regulatory genes for prepubertal oocyte maturation, including GTPBP4, TOMM7, CKS2, CCP110, CKAP5, ESCO1, CENPE, CENPF, CENPN, and SGO2.
- Demonstrated that GTPBP4 significantly enhances prepubertal goat oocyte developmental competence by promoting cell cycle progression, organelle maturation, and mRNA translation.
Conclusions:
- Detailed molecular map of goat oocyte maturation, highlighting differences between adult and prepubertal stages.
- Identified GTPBP4 as a crucial factor for enhancing prepubertal oocyte competence.
- Findings support potential fertility preservation strategies for prepubertal girls and advancements in domestic animal breeding.
