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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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A single-cell transcriptomic atlas of sensory-dependent gene expression in developing mouse visual cortex.

Andre M Xavier1, Qianyu Lin1, Chris J Kang1

  • 1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

Development (Cambridge, England)
|February 28, 2025
PubMed
Summary

Sensory experience shapes brain development by altering gene expression in visual cortex cells. This study identified key genes in excitatory neurons that influence synaptic connections and circuit wiring during critical developmental periods.

Keywords:
Cell signalingCircuit developmentNeuronSensory experienceSynapseTranscriptionVisual cortex

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Molecular Biology

Background:

  • Postnatal brain development relies on sensory experience to mature neural circuits.
  • The precise molecular mechanisms underlying sensory-dependent circuit remodeling are not fully understood.
  • Gene expression changes driven by sensory input are hypothesized to mediate circuit refinement.

Purpose of the Study:

  • To identify genes regulated by sensory experience in the developing mouse visual cortex.
  • To investigate the cell-type-specific transcriptional responses to sensory input or deprivation.
  • To uncover molecular mediators of sensory-dependent synaptic development and circuit wiring.

Main Methods:

  • Generated a single-nucleus RNA sequencing dataset from mouse visual cortex.
  • Analyzed transcriptional responses in 118,529 nuclei across 16 cell types.
  • Compared gene expression profiles from control, sensory-deprived, and sensory-stimulated mice.

Main Results:

  • Identified 1268 sensory-induced genes in the developing brain.
  • Observed robust transcriptomic changes in layer 2/3 excitatory neurons.
  • Discovered that sensory-induced genes in these neurons enhance synapse-to-nucleus communication and axon guidance.

Conclusions:

  • Sensory experience profoundly influences gene expression in the developing visual cortex.
  • Layer 2/3 excitatory neurons are key players in sensory-driven circuit maturation.
  • This dataset provides insights into the molecular basis of how sensory input shapes neural circuit wiring.