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Updated: Jul 12, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
Mechanistic insights into chloroethene dechlorination by Dehalococcoides mccartyi strain CWV2: A multi-omics
Chih-Ming Kao1, Ku-Fan Chen2, Po-Sheng Kuo3
1Institute of Environmental Engineering, National Sun Yat-Sen University, Kaohsiung, 80424, Taiwan.
Abstract:
This study provides an in-depth investigation of the novel Dehalococcoides mccartyi (Dhc) strain CWV2, isolated from Taiwan, for its effectiveness in dechlorinating various chloroethenes, including PCE, TCE, DCEs, and VC, to ethene. Through multi-omics analyses, including genomic, transcriptomic, translatomic and proteomic profiling, we uncovered the mechanisms behind TCE dechlorination by strain CWV2. The genomic analysis identified key reductive dehalogenase (RDase) genes, pceA and vcrA, which enhance our understanding of the versatile dechlorination pathways in Dhc. Ribosome profiling provided detailed insights into the translational regulation of vcrA, revealing sophisticated genetic control over protein synthesis. Complementary BN-PAGE and proteomic analyses identified key RDase VcrA, offering further insights into the activity of the organohalide respiration (OHR) complex within CWV2 and its metabolic pathways. Multi-omics analyses provide a comprehensive understanding of the mechanisms behind TCE dechlorination and organohalide respiration, offering valuable insights to advance bioremediation strategies for chloroethene-contaminated environments.
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