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A mitochondria targeted hydrogen peroxide fluorescent probe for monitoring oxidative stress during influenza virus
Hao Chang1, Jianing Wang2, Yang Yang2
1Department of Laboratory Medicine, The Second Clinical Medical College, Jinan University (Shenzhen People's Hospital), Shenzhen, Guangdong 518020, China; Integrated Chinese and Western Medicine Postdoctoral Research Station, Jinan University, Guangzhou 510632, China.
Abstract:
Hydrogen peroxide (H2O2), an important marker of oxidative stress, plays a significant role in infectious diseases. Oxidative stress induced by influenza virus infection is intricately linked to the pathological processes of host cells. In this work, a fluorescent probe QLC1 based on the coumarin was developed for fluorescence imaging of mitochondrial H2O2 level in host cells during influenza virus infection. The self-immolative reaction of QLC1 triggered by H2O2 will lead to a 250-fold fluorescence enhance and the limit of detection was determined to be 0.176 μM. Using this probe, we monitored oxidative stress during influenza infection and identified a link between redox status and influenza virus replication.
Insights
Researchers developed a fluorescent probe, QLC1, to measure hydrogen peroxide (H2O2) in cells during influenza virus infection. This tool revealed a connection between cellular redox status and viral replication, aiding in understanding infectious disease pathology.
Area of Science:
- Biochemistry
- Cell Biology
- Infectious Diseases
Background:
- Hydrogen peroxide (H2O2) is a key indicator of oxidative stress, significantly impacting host cell pathology during infectious diseases like influenza.
- Influenza virus infection induces oxidative stress, which is closely associated with the disease's pathological mechanisms.
Purpose of the Study:
- To develop a novel fluorescent probe for visualizing mitochondrial H2O2 levels in host cells during influenza virus infection.
- To investigate the relationship between cellular redox status and influenza virus replication using the developed probe.
Main Methods:
- Development of a coumarin-based fluorescent probe (QLC1) designed for H2O2 detection.
- Utilizing the probe for fluorescence imaging of mitochondrial H2O2 in host cells infected with influenza virus.
- Quantifying H2O2 levels and assessing the limit of detection (0.176 μM).
Main Results:
- The QLC1 probe exhibited a 250-fold fluorescence enhancement upon reaction with H2O2.
- Mitochondrial H2O2 levels were successfully monitored in host cells during influenza infection.
- A direct correlation was identified between the cellular redox state and the replication efficiency of the influenza virus.
Conclusions:
- The developed fluorescent probe QLC1 is effective for monitoring mitochondrial H2O2 levels and cellular oxidative stress in the context of influenza virus infection.
- Findings highlight the critical role of redox status in regulating influenza virus replication, offering potential therapeutic targets.
