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Peritoneal Dialysis II: Peritoneal Dialysis Systems and Complications01:25

Peritoneal Dialysis II: Peritoneal Dialysis Systems and Complications

Peritoneal dialysis (PD) is a medical process that removes waste products and excess fluid from the body using the peritoneal membrane as a natural filter.Peritoneal Dialysis MethodsSeveral methods can be used for peritoneal dialysis, including Acute Intermittent Peritoneal Dialysis, Continuous Ambulatory Peritoneal Dialysis, and Automated Peritoneal Dialysis, also known as Continuous Cyclic Peritoneal Dialysis.Acute Intermittent Peritoneal Dialysis (AIPD) is used for patients with uremic...

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Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity
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Plasma-activated solutions prevent peritoneal adhesion formation by regulating eNOS expression in mesothelial cells.

Yuyi Ma1, Tuanhe Sun2, Kaijie Ren1

  • 1Department of Surgical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.

Journal of Advanced Research
|February 28, 2025
PubMed
Summary

Plasma-activated solutions (PAS) effectively prevent peritoneal adhesions by restoring eNOS expression, reducing oxidative stress, apoptosis, and mesothelial-to-mesenchymal transition. This novel therapy shows promise for clinical translation in adhesion prevention.

Keywords:
MMTPlasma-activated solutionsPostoperative peritoneal adhesionsRedox homeostasiseNOS

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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Surgical Innovation

Background:

  • Peritoneal adhesions cause significant morbidity with limited therapeutic options.
  • Current adhesion prevention strategies have inconsistent efficacy and potential side effects.
  • Plasma-activated solutions (PAS) show potential for anti-inflammatory and healing effects, but their role in adhesion prevention requires investigation.

Purpose of the Study:

  • To investigate the therapeutic potential of PAS in preventing peritoneal adhesion formation.
  • To elucidate the underlying mechanisms of PAS in preventing peritoneal adhesions.

Main Methods:

  • Established murine models for peritoneal adhesions (ischemic button and cecum-peritoneum abrasion).
  • In vitro studies using human peritoneal mesothelial cells treated with LPS or TGF-β1 to model apoptosis and mesothelial-to-mesenchymal transition (MMT).
  • Quantified apoptosis, ROS levels, MMT markers, and inflammatory cytokines; performed histological evaluations.

Main Results:

  • PAS significantly reduced adhesion scores in both murine models (p < 0.01).
  • In vitro, PAS decreased mesothelial cell apoptosis (p < 0.01) and suppressed MMT markers (N-cadherin, Vimentin; p < 0.05).
  • PAS attenuated oxidative stress, including general ROS (p < 0.001) and mitochondrial ROS (p < 0.01), by restoring eNOS expression via reactive nitrogen species (RNS).

Conclusions:

  • PAS effectively prevents peritoneal adhesions through RNS-mediated eNOS restoration.
  • PAS suppresses key mechanisms including oxidative stress, apoptosis, and MMT.
  • PAS represents a novel and promising therapeutic strategy for adhesion prevention with potential for clinical translation.