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Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

Published on: February 4, 2013

Two colorimetric LAMP systems for nucleic acid-based diagnostics.

Antao Sun¹, Petra Stejskalová², Xiaocheng Liu¹

¹Ministry of Education Key Laboratory of Micro and Nano Systems for Aerospace, School of Mechanical Engineering, Northwestern Polytechnical University, 127 West Youyi Road, 710072, Xi'an, Shaanxi, PR China.

Analytica Chimica Acta

|February 28, 2025

Summary

This summary is machine-generated.

This study presents a new 8-well colorimetric loop-mediated isothermal amplification (LAMP) system for rapid SARS-CoV-2 detection. The system demonstrates comparable precision to fluorescence-based methods, offering an accessible diagnostic tool.

Keywords:

Biosensors and actuators Colorimetric detection Loop-mediated isothermal amplification (LAMP)Point-of-care diagnostics Rapid testing technologies SARS-CoV-2 diagnostics

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Area of Science:

Molecular Biology
Biotechnology
Medical Diagnostics

Background:

Development of advanced diagnostic tools for infectious diseases.
Need for rapid, accessible, and simple SARS-CoV-2 detection methods.
Limitations of traditional fluorescence-based assays.

Purpose of the Study:

To design and evaluate an automated 8-well colorimetric loop-mediated isothermal amplification (LAMP) system for SARS-CoV-2 detection.
To compare the performance of two colorimetric LAMP configurations against traditional fluorescence-based methods.
To assess the system's rapidity, precision, and simplicity.

Main Methods:

Assembly and testing of two distinct colorimetric LAMP systems utilizing different LED/sensor configurations.
Comparison with fluorescence-based loop-mediated isothermal amplification (LAMP) on a commercial qPCR instrument.
Validation using varying SARS-CoV-2 RNA concentrations.

Main Results:

Colorimetric RT-LAMP assays achieved critical threshold time (C_T) values comparable to fluorescence-based detection.
Consistent and precise C_T values were observed across a range of viral loads (approx. 1570 to 157,000 copies·μL^-1).
The system demonstrated rapid response and precision, highlighting its simplicity.

Conclusions:

The developed colorimetric LAMP system offers a rapid, precise, and accessible diagnostic tool for SARS-CoV-2.
Its potential for point-of-need diagnostics is significant for pandemic response.
The system's adaptability may extend to detecting other pathogens.