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Updated: May 24, 2025

A FACS-based Protocol to Isolate RNA from the Secondary Cells of Drosophila Male Accessory Glands
Published on: September 5, 2019
AgRP neurons shape the sperm small RNA payload
Iasim Tahiri1,2, Sergio R Llana1, Francisco Díaz-Castro1,3
1Neuronal Control of Metabolism, Institut d'Investigacions Biomèdiques August Pi i Sunyer, Rosselló 149-153, 5th floor, Barcelona, 08036, Spain.
Abstract:
Paternal dietary patterns and obesity can affect offspring through epigenetic signals in sperm RNA. By activating hypothalamic Agouti-related peptide (AgRP) neurons in mice to simulate obesity's effects, we observed changes in the small noncoding RNA payload of sperm. These alterations, particularly in transfer RNA-derived small RNAs (tsRNAs), are similar to those induced by short-term high-fat diets, suggesting a common upstream regulatory mechanism involving AgRP neurons that affects metabolic epigenetic inheritance.
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