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Related Experiment Video

Updated: May 24, 2025

Production of Large Numbers of Size-controlled Tumor Spheroids Using Microwell Plates
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High-Throughput Programmable Tumor Spheroid Generation Using the Magneto-Archimedes Effect.

Xiangxiang Zhang1, Chao Li1, Shubin Li1

  • 1State Key Laboratory of Urban Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, 92 West Da-Zhi Street, Harbin 150001, China.

ACS Applied Materials & Interfaces
|March 1, 2025
PubMed
Summary

This study presents a cost-effective, noninvasive method using the magneto-Archimedes effect to create 3D tumor spheroids for drug discovery and tissue engineering. These spheroids mimic real tissues and enable advanced cancer research.

Keywords:
drug screeninghybrid structuremagneto-Archimedes effecttissue transplantingtumor spheroid

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Related Experiment Videos

Last Updated: May 24, 2025

Production of Large Numbers of Size-controlled Tumor Spheroids Using Microwell Plates
10:44

Production of Large Numbers of Size-controlled Tumor Spheroids Using Microwell Plates

Published on: November 18, 2013

17.4K
Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening
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Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening

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Generation of 3D Tumor Spheroids for Drug Evaluation Studies
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Generation of 3D Tumor Spheroids for Drug Evaluation Studies

Published on: February 24, 2023

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Area of Science:

  • Biotechnology
  • Biomedical Engineering
  • Cancer Research

Background:

  • Three-dimensional (3D) tumor spheroids mimic in vivo tumor microenvironments, crucial for drug discovery and tissue engineering.
  • Existing methods for spheroid generation can be time-consuming, costly, or invasive.

Purpose of the Study:

  • To develop a simple, cost-effective, and noninvasive high-throughput method for producing 3D tumor spheroids.
  • To demonstrate the utility of these spheroids in anticancer drug screening, tumor inoculation, and studying cell-tissue interactions.

Main Methods:

  • Utilized the magneto-Archimedes effect for spheroid formation.
  • Employed different templates and cell ordering for versatile spheroid morphologies and spatial coding.
  • Validated spheroid similarity to mouse homograft tumors via immunofluorescence.

Main Results:

  • Successfully produced versatile 3D tumor spheroids with high-throughput capability.
  • Demonstrated efficacy of Temozolomide (TMZ) over 5-FU for glioma treatment using these spheroids.
  • Showcased successful tumor growth in mice from C6 spheroids and induced apoptosis in tumor cells via reactive oxygen species (ROS) diffusion from artificial cell aggregates.

Conclusions:

  • The magneto-Archimedes effect offers a scalable and efficient approach for generating 3D tumor spheroids.
  • These spheroids are valuable tools for anticancer drug screening, in vivo tumor modeling, and understanding intercellular signaling.
  • The method holds significant potential for complex tissue engineering and advanced cancer research applications.