Construction, characterization, and properties of a probiotic delivery system based on oxidized high amylose starch

This study demonstrates the preparation of oxidized high amylose starch (OHAS) and its effect on the survival and release of Lacticaseibacillus rhamnosus GG (LGG) in the gastrointestinal tract when combined with sodium alginate (SA). OHAS with different degrees of oxidation was combined with SA, and through rheological analysis, an SA/OHAS composite with good viscoelasticity, stable structure, and favorable for probiotic colonization was selected as the probiotic carrier. Specifically, SA/OHAS showed a good encapsulation efficiency of 46.21 % and maintained good viability after gastric juice digestion, with a surviving probiotic count of 5.87 × 10⁶ CFU/mL. It exhibits good release efficiency in intestinal fluid, with a release rate reaching 85.67 % within 2 h. In vivo intestinal transit visualization evaluation after feeding mice showed that SA/OHAS could effectively prolong the retention time of probiotics in the intestine. In vitro experiments further proved that SA/OHAS could significantly enhance the resistance of probiotics in gastric juice and provide a targeted release for intestine-specific delivery. These results provide a promising strategy for the delivery system of SA/OHAS encapsulated probiotics in oral food applications.