Related Experiment Video For Cancer
Updated: May 24, 2025

17:23
Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works
Published on: November 30, 2009
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Protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo chorioallantoic membrane
Tommy Chastel1, Serena Filiberti2, Stefania Mitola2
1Tumor Microenvironment and Resistance to Treatment Lab, INSERM U1194, 34090 Montpellier, France.
STAR Protocols
|March 1, 2025
Abstract:
The chick embryo chorioallantoic membrane (CAM) is a viable alternative in vivo model to explore cancer biology due to its simplicity, accessibility, and cost-effectiveness. Here, we present a protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo CAM model. We describe steps for initial general preparation and for the angiogenic and tumorigenic assays in two distinct sections.

