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Protocol for converting assigned nuclear Overhauser enhancement spectroscopy series peaks into exact distance

Morkos A Henen1, David McCall2, David Jones1

  • 1University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, USA.

STAR Protocols
|March 1, 2025
PubMed
Summary

This study details a protocol for extracting precise interatomic distance restraints from Nuclear Magnetic Resonance (NMR) Nuclear Overhauser Effect Spectroscopy (NOESY) spectra. This method enhances biomolecular structure calculations using the exact Nuclear Overhauser Effect (eNOE) approach.

Keywords:
BiophysicsChemistryProtein BiochemistryStructural Biology

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