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Enhancing prostate cancer cells' sensitivity to flutamide by resveratrol: An in-vitro study
Seyedeh Fatemeh Hosseini1, Seyed Reza Yahyazadeh2, Akram Mirzaei1
1Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Tissue & Cell
|March 1, 2025
Summary
Resveratrol enhances flutamide treatment for prostate cancer by reducing required dosage and improving efficacy. This natural compound promotes apoptosis and inhibits cancer cell growth, offering a promising strategy for managing prostate cancer.
Area of Science:
- Oncology
- Pharmacology
- Natural Products Research
Background:
- Prostate cancer is the most common cancer in men, necessitating novel treatment strategies.
- Current treatments face challenges in efficacy and side effects.
- Investigating natural compounds like resveratrol offers potential therapeutic benefits.
Purpose of the Study:
- To evaluate resveratrol's potential to enhance flutamide efficacy in prostate cancer.
- To assess the impact of resveratrol on prostate cancer cell response to flutamide.
- To determine if resveratrol can minimize adverse effects associated with flutamide treatment.
Main Methods:
- Utilized MTT assays to determine IC50 values for resveratrol and flutamide.
- Employed Annexin-V/PI and Hoechst staining for apoptosis and nucleus morphology analysis.
- Conducted real-time PCR to analyze gene expression related to apoptosis, EMT, and angiogenesis.
Main Results:
- Combined resveratrol and flutamide reduced prostate cancer cell migration, proliferation, and colony formation.
- Resveratrol upregulated apoptosis-related genes (BAX) and influenced BCL-2 levels.
- The combination therapy altered expression of genes involved in angiogenesis (VEGFC) and epithelial-mesenchymal transition (EMT).
Conclusions:
- Resveratrol reduced the effective dose of flutamide for prostate cancer cell lines.
- Resveratrol improved flutamide's side effect profile and increased cellular sensitivity.
- This combination therapy presents a promising approach for prostate cancer treatment.
