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Updated: May 24, 2025
Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells
Published on: November 17, 2017
Effect of cold plasma on ochratoxin A detoxification: Response surface, degradation pathways, and cytotoxicity
Jiarong Zheng1, Luling Zhao1, Jianhao Zhang1
1Sanya Institute of Nanjing Agricultural University, Sanya 572025, China; National Center of Meat Quality and Safety Control, Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Abstract:
Ochratoxin A (OTA) is one of the most widely contaminated mycotoxins. Dielectric barrier discharge cold plasma (DBD-CP) holds great potential in mycotoxin degradation. This study aimed to investigate the impact of DBD-CP on OTA degradation, assess the cytotoxicity of its degradation products, and evaluate its influence on wheat quality. Under the optimized DBD-CP conditions, a significant OTA degradation of 94.2 % was achieved within a short treatment time. Five major OTA degradation products were produced after DBD-CP treatment through various reaction pathways, including hydroxylation/dehydroxylation, demethylation, dechlorination, esterification, decarboxylation, and lactone opening, namely C19H17NO7 (P1), C18H17NO5 (P2), C21H21NO7 (P3), C20H19NO6 (P4), and C19H19NO6 (P5). HepG2 cell viability was increased from 50.8 % to 94.7 % after a treatment duration of 3.60 min. Furthermore, DBD-CP removed 71.4 % of OTA from wheat without significantly affecting other physiological properties. Hence, DBD-CP is a promising effective method for OTA detoxification in wheat to ensure human and animal health.
Insights
Dielectric barrier discharge cold plasma effectively degrades Ochratoxin A (OTA), a common mycotoxin. This method also reduces OTA
Area of Science:
- Food Science and Technology
- Biotechnology
- Analytical Chemistry
Background:
- Ochratoxin A (OTA) is a prevalent mycotoxin contaminating food and feed globally.
- Mycotoxin contamination poses significant risks to human and animal health.
- Conventional detoxification methods often impact food quality or are inefficient.
Purpose of the Study:
- To investigate the efficacy of dielectric barrier discharge cold plasma (DBD-CP) in degrading OTA.
- To assess the cytotoxicity of OTA degradation products.
- To evaluate the impact of DBD-CP treatment on wheat quality.
Main Methods:
- Optimized DBD-CP treatment parameters were applied to OTA.
- Degradation products were identified using analytical techniques.
- Cytotoxicity was assessed using HepG2 cell viability assays.
- Wheat samples were analyzed for OTA content and physiological properties post-treatment.
Main Results:
- Achieved 94.2% OTA degradation under optimized DBD-CP conditions.
- Identified five major OTA degradation products formed via multiple reaction pathways.
- Significantly reduced cytotoxicity, increasing HepG2 cell viability from 50.8% to 94.7%.
- Removed 71.4% of OTA from wheat with minimal impact on overall quality.
Conclusions:
- DBD-CP is a highly effective and rapid method for OTA detoxification.
- The process reduces the toxicity associated with OTA contamination.
- DBD-CP offers a promising approach for ensuring the safety of wheat and other commodities.
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