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  11. Development Of Ph-adjusted Nmr Methodology For Quantitation Of Caffeoylquinic Acid Derivatives And Evaluation Of Their Antimalignant Pleural Mesothelioma Potential.
  1. Home
  3. Research Domains
  5. Chemical Sciences
  7. Analytical Chemistry
  9. Quality Assurance, Chemometrics, Traceability And Metrological Chemistry
  11. Development Of Ph-adjusted Nmr Methodology For Quantitation Of Caffeoylquinic Acid Derivatives And Evaluation Of Their Antimalignant Pleural Mesothelioma Potential.

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Development of pH-adjusted NMR methodology for quantitation of caffeoylquinic acid derivatives and evaluation of their antimalignant pleural mesothelioma potential.

Hau Thi Hong Bui1, Yoon-Jin Lee2, Trung Huy Ngo1

  • 1College of Pharmacy, Yeungnam University, Gyeongsan-si, Gyeongsangbuk-do 38541, Republic of Korea.

Journal of Pharmaceutical and Biomedical Analysis
|March 2, 2025

View abstract on PubMed

Summary
This summary is machine-generated.
Keywords:
(1)H iterative full spin analysisCaffeoylquinic acidsCuscuta japonicaGreen coffee bean

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A new pH-adjusted quantitative NMR method accurately measures caffeoylquinic acids (CQAs). Certain CQAs from Cuscuta japonica show potential anticancer effects against malignant pleural mesothelioma cells.

Area of Science:

  • Analytical Chemistry
  • Natural Product Chemistry
  • Pharmacology

Background:

  • Quantitative analysis of caffeoylquinic acids (CQAs) is complex due to structural similarities and instability.
  • Existing methods face challenges with isomerization and degradation of CQAs.
  • Accurate quantification is crucial for understanding CQA content in botanical extracts.

Purpose of the Study:

  • To develop a robust quantitative method for analyzing CQAs in plant extracts.
  • To evaluate the anticancer potential of Cuscuta japonica extract and its isolated CQAs.
  • To compare quantum mechanics (QM)-based and non-QM-based quantitative NMR (qHNMR) approaches.

Main Methods:

  • A pH-adjusted quantitative 1H NMR (qHNMR) method using a MeOD/HEPES-d18 buffer was developed.
Malignant pleural mesothelioma
Quantitative NMR
pH
  • The method was applied to quantify chlorogenic acid and 3,5-di-CQA in Cuscuta japonica extract.
  • Quantification of chlorogenic acid in green coffee bean extracts was performed.
  • Anticancer activity was assessed against NCI-H2452 cells.

    • Main Results:

    • The pH-adjusted qHNMR method demonstrated effectiveness in quantifying CQAs.
    • QM-qHNMR improved the precision and sustainability of quantitative NMR analysis.
    • Cuscuta japonica extract and isolated CQAs (1-7) were tested for anticancer effects.
    • Methyl di-CQA esters (5 and 7) significantly inhibited proliferation and induced apoptosis in NCI-H2452 cells.

    Conclusions:

    • A novel pH-adjusted qHNMR method offers improved accuracy for CQA quantification.
    • QM-qHNMR presents a more precise and sustainable approach for quantitative NMR.
    • Methyl di-CQA esters from Cuscuta japonica exhibit promising therapeutic potential for malignant pleural mesothelioma.
    • Further research into these compounds could lead to new anticancer treatments.