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Updated: May 24, 2025

Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane SSM-Based Electrophysiology
Published on: May 3, 2021
Allosteric modulation of protein-protein interactions in signal transduction with Nanobodies
Els Pardon1, Alex Wohlkönig1, Jan Steyaert1
1VIB-VUB Center for Structural Biology, VIB, Pleinlaan 2, Brussels, Belgium; Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, Brussels, Belgium.
Abstract:
Nanobodies (Nbs), the variable domains of heavy-chain only antibodies that naturally occur in camelids, are exquisite molecular tools to stabilize dynamic proteins in unique functional conformations. Recent developments in Nb discovery allow to select allosteric Nbs that perturb the distribution of conformational ensembles of protein complexes that mediate signaling, leading to the allosteric modulation of the signals they transmit. Evidence is also accumulating that such conformational specific Nbs do not stabilize new conformational states but rather change the distribution of existing states to allosterically induce transitions, imprinted by the natural ligands of the system.
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