Sphingosinicella rhizophila sp. nov., Isolated from Oat (Avena sativa L.) Rhizosphere Soil

A Gram-stain-negative, aerobic, rod-shaped, and yellow-colored bacterium, designated strain GR2756^T, was isolated from a rhizosphere soil sample of oat (Avena sativa L.). The strain GR2756^T was able to grow at 15-35 °C, pH 6.0-8.0, and 0-1.5 % (w/v) NaCl concentration. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain GR2756^T was clustered with Sphingosinicella vermicomposti YC7378^T (95.7 %), Sphingosinicella humi QZX222^T (95.6 %), and Sphingomonas montis DRJ-4^T (95.5 %). Strain GR2756^T contained ubiquinone Q-10 and homospermidine as the major respiratory quinone system and polyamine, respectively. The major fatty acids detected from strain GR2756^T were C_16:0, Summed Feature 3 (C_16:1 ω7c and/or C_16:1 ω6c), and Summed Feature 8 (C_18:1 ω7c and/or C_18:1 ω6c), C_17:1 ω6c and C_16:0 2-OH. The major polar lipids are phosphatidylethanolamine (PE), phospholipid, glycolipid, and unidentified polar lipid. The genomic DNA G+C was 63.2%. Between the strains GR2756^T and S. humi CCTCC AB 2018030^T, the ANI and dDDH values were lower than the proposed and generally accepted species boundaries. On the basis of phenotypic, genotypic, and phylogenetic analysis, strain GR2756^T represents a novel species of the genus Sphingosinicella, for which the name Sphingosinicella rhizophila sp. nov. is proposed. The type strain of the proposed new taxon is strain GR2756^T (= CCTCC AB 2023102^T = CGMCC 1.61580^T = NBRC 116723^T).